The biocrystallization method has been used as a holistic method for evaluating food samples since the 1930s and has, as recent reviews have concluded, been successful in discriminating farming systems in several studies. However, it is noted that a standardization and validation for more objective means of evaluation of biocrystallization pictures and a clearly defined and communicable language, as well as possibilities for verification, are still lacking. During the years 2002–2006 a project for standardizing and validating the visual evaluation of these biocrystallizations took place, performed by a group consisting of three European research institutes. As no pre-set methodology nor norms for the visual evaluation existed, the choice was made to work according to existing norms on sensory analysis and to adapt these to the visual evaluation. The main norm, ISO-Norm 11035, for establishing sensory profiles was adapted towards these pictures. A panel was formed, mainly morphological descriptors were selected and defined, a scale with references was established, the panel trained and tested for pictures, produced on the basis of carrots of different qualities. Reliability and validity aspects of the panel, as well as the structure of the set of criteria were evaluated. According to standard rates for diverse statistical tests the panel has been validated for the evaluation of carrots with a defined set of descriptors. The set of descriptors contains sub-domains which can be used for further development of the evaluation method, towards a more integrated, holistic ‘Gestalt’-evaluation, and towards finally interpretation of these pictures. The followed process and results are unique in connection to this method.